N.B. expands COVID-19 wastewater monitoring to 4 more cities

New Brunswick has expanded its COVID-19 wastewater monitoring to four more cities.

Saint John, Bathurst, Campbellton and Miramichi were added to the national COVID-19 wastewater surveillance dashboard on Tuesday, with their most recent test results dating frombetween March 20 and March 27.

Moncton andFredericton were previously included.

Wastewater results can serve as an early warning of increased community infection levels and risks.

People who are infected shed the virus in their feces in the form of a genetic material called ribonucleic acid, or RNA, before they show symptoms of COVID-19. This can be found in raw sewage and can detect the virusin a community up to 10 days prior to clinical testing, according to the Public Health Agency of Canada.

Now that New Brunswick has further restrictedPCRtesting, requiring a referral from a health-care provider, some experts have urged an increase in wastewater monitoring to track virus spread and any new variants.

Data for the four newly added cities shows a recent spike in viral load for Miramichi, a slight increase forCampbellton, a levelling off following a jump for Saint John, and slight decrease for Bathurst.

Moncton and Fredericton, meanwhile, both sawrecent drops in viral loads, according to the dashboard.

Overall, the results appear to be relatively low compared to some other jurisdictions, and compared to earlier in the pandemic. More details are included at the end of the story.

In the works for months

The Department of Health told CBC in January that testing in Saint John and Bathurstwas coming "in the near future."

"It takes time for sites to get set up, as there is new equipment, processes and procedures for project participants to navigate," department spokesperson Sean Hatchard said Wednesday in an emailed statement.

Aboutthree to four weeks of COVID-19 wastewater data are needed for "meaningful trend results," saidPublic Health Agency of Canada spokespersonAnna Maddison. Saint John and Bathurst have now met therequirementand are included," she said.

Hatchard did not respond to questions about how or why Campbellton and Miramichi were selected, or what, if any other sites are planned.

Delayed results

The Department of Health did not respond when asked how useful it is to posttest results as much as 15days after the fact, such as the case in Miramichi, if they do show increased levels, and whether that's too late for people to try to protect themselvesfrom potentially being exposed to people who were infectious all that time.

The Public Health Agency of Canada spokespersondid not answer directly.

"We are constantly working to improve this process to ensure results are available as soon as possible," saidMaddison.

Public Health Agency of Canada's National Microbiology Laboratoryin Winnipeg is "consistently optimizing its workflows to make data more readily available," she said.

"We are also routinely communicating with our partners to address any delays in sample transport to help ensure samples arrivein our laboratory as soon as possible."

Asked why some other sampling sites, such as Vancouver and Toronto,have more timely results than most of those in New Brunswick, Maddisonsaid the differences in reporting times can be due to the time it takes to shipand transportsamples to the national lab.

"On average, the shipping time for these [New Brunswick] sites is a day or two longer than other sites, which can contribute to the delays in reporting," she said in an emailed statement.

In addition,if samples arrive on a Friday or duringthe weekend, it can further delay processing and testing, Maddison said.

The lab maintains a consistent three-day turnaround time between sample receipt and results, she said.

"However, due to the complex nature of wastewater samples, sometimes retesting of samples is required to ensure the utmost confidence in the results, which adds more time to the process."

The Health Department has said wastewater samples aretested at the Dr. Georges-L.-Dumont University Hospital Centre laboratory in Moncton andare also being sent to the nationallabfor "validation"and sequencing.

As to why New Brunswick samples need to be tested twice, Maddison said the national lab provides reference services to all provinces and territories for COVID-19 and other pathogens, including "confirmatory testing, quality assurance, and in-depth analysis of difficult-to-diagnose specimens."

The results posted on the dashboard are those generated by the national lab, she said.

Future of public health surveillance

Public Health Agency of Canadacontinues to collaborate with other federal departments, provincial, territorial and municipal governments and academics across the country to expand the wastewater surveillance dashboard to provideadditional data to citizens, including additional cities, she said.

"Knowing the daily or weekly changes in the amount of infectious disease detected in wastewater can help manage outbreaks (through public health action such as vaccination and testing) as well as health care resource allocation (such as hospital beds and staffing needs)," Maddisonsaid.

The selection of sites for wastewater sampling and the decision to release results is at the discretion of provinces, territories, and municipalities, she added.

Detailed results

In Miramichi, the seven-day rolling average of COVID-19 viral load, expressed as the number of viral gene copies found in a millilitre of raw sewage, was 51, as of March 20, the most recent results available. That's up from 19 copies/mLon March 16.

By comparison, the level in Alberton, P.E.I., was more than three times higher, at 165copies/mL on March 22. Some jurisdictions, such as Vancouver's Lulu Island, have seen readings over 1,000 copies/mlduring the peak of the pandemic.

Campbellton's most recent level was six copies/mL, as of March 24, up from two on March 20.

Levels in Saint John haveremained below one since it started testing on March 2. On March 27, the viral loadwas 0.16,unchanged from four days earlier, when it had jumped from 0.005.

In Bathurst, the seven-day rolling average was nine copies/mL, as of March 21, down from 11 the two previous readings.

Moncton's latest reading was 12 copies/mL, as of March 23,down from 24 and two previous readings of low-60s mid-month.

Similarly, Frederictonhad a seven-day rolling average of 15 copies/mL on March 23, down from 20 and 46.